The murine homologous macrophage hybridoma cell lines were obtained by somatic cell fusion between Picibanil-elicited peritoneal macrophages and HAT-sensitive marine myelomonocytic cell lice, P388D1-S. The hybridoma cell lines that were grown in vitro for more than a year were confirmed by the expression of phagocytosis, adherence, monocyty specific nonspecific esterases, H-2 antigen, karyotypes, and other cell surface markers. In addition to the characterization of hybridoma cells. I investigated their ability to produce cytokines. After stimulation with LPS, supernatants of hybridoma clones showed IL-I and IL-i inhibitor activities. This study shows that homologous somatic cell fusion with marine macrophages can be used to define heterogeneous macrophage functions and gene expressions at the clonal level.
Author thanks Dr.Y.H.Park for his help to use the liquid Scintillation counter at Veterinary Research Institute, Anyang.
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